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EMB (Levine) agar
EMB Agar, originally recommended by Levine, is used to isolate and identify enterobacteria (notably Escherichia coli and Enterobacter aerogenes) in pharmaceutical, cosmetic and food products as well as water. It is also used as a confirmation media for Escherichia coli in cosmetic products.
The typical composition reflects that defined in the standard NF EN ISO 21150.
Available in dehydrated medium 500g container.
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Eosin Y and Methylene blue have low selective capacities, since they only partially inhibit the development of Gram-positive bacteria such as enterococci. The dyes allow to the differentiation between lactose-positive and lactose-negative bacteria. Coliform strains form violet to brown colonies, while salmonellae are colorless, transparent or amber.
Escherichia coli present a metallic sheen under oblique lighting.
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