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EC broth
EC Broth (abbreviation for Escherichia coli) is a selective media used for the confirmation of E. coli in water, milk and other food products, as well as live shellfish.
The typical composition of the broth responds to that defined in the normalized method of presumptive Escherichia coli enumeration, MPN technique (NF ISO 7251).
Available as dehydrated medium: 500g package.
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
The media corresponds to the buffered lactose broth, with added bile salts, whose objective is to inhibit the development of spore-formers and enterococci, while at the same time promoting Escherichia coli growth.
The development of coliforms and notably E. coli is demonstrated through the appearance of turbidity associated with gas production in a Durham tube following lactose fermentation.
