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Azide Dextrose Broth (Acc. to Rothe)
Azide Dextrose Broth (Rothe) is used for the enumeration of enterococci in drinking water, frozen foods and other food products by the most probable number method.
Available as dehydrated medium: 500g bottle
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
The high nutritive capacity of Azide Dextrose Broth is due to the presence of a high concentration of polypeptone and glucose.
Sodium chloride maintains the osmotic equilibrium.
Potassium phosphates buffer the medium.
Sodium azide inhibits the growth of Gram-negative bacteria by its bacteriostatic action and favors that of fecal streptococci.
After observing cultures in tubes (presumptive test), it is necessary to carry out a confirmation in Ethyl violet Azide Broth (Litsky broth).
