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Bromocresol Purple Lactose Broth
Bromocresol Purple (BCP) Lactose broth is used as enrichment media for the enumeration of coliforms and of thermotolerant coliforms in water, according to the standard NF T90-413 (MPN method).
Dehydrated medium: 500 g bottle
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
The media is used in single and in double strength varieties.
Tryptone and meat extract supply nitrogen nutrients required for bacterial growth.
The fermentation of lactose is shown by the acidification of the medium that causes the pH indicator (bromocresol purple) to turn yellow, as well as by gas production in Durham tubes.
In order to confirm the presence of coliform bacteria, subcultures must be prepared using the appropriate confirmation media.