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Listeria Enrichment Broth (Modified UVM I)
The enrichment broths UVM (University of Vermont) involve a 2 step process, leading to a higher degree of isolation of Listeria monocytogenes in meat products (including poultry).
UVM 1 enrichment broth allows for primary enrichment of Listeria.
Dehydrated medium : 500 g bottle
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Peptones, yeast extract and meat extract supply the essential nutrients for the growth of Listeria.
The sodium chloride concentration increases selectivity.
Phosphates buffer the pH of the medium.
Esculin is hydrolyzed to glucose and esculetin by Listeria.
Nalidixic acid blocks the DNA replication of bacteria sensitive to this antibacterial agent.
Accompanying Gram-positive microflora are inhibited by acriflavin.
