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MacConkey Sorbitol (CT-SMAC) Agar
MacConkey Sorbitol (CT-SMAC) agar is a selective media used for the isolation and differentiation of Escherichia coli O157 in water, milk, beef products and other food preparations.
Available as dehydrated base medium: 500 g bottle.
To be used with the Cefixime-Tellurite Selective supplement (BS03708).
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Tryptone and Peptic digest of meat favor the growth of Escherichia coli O157.
Sorbitol-negative microorganisms (notably O157) produce transparent colonies.
Sorbitol-positive microorganisms are detected by the colonies turning red (under the action of neutral red).
Secondary microflora are inhibited by the association between the bile salts, crystal violet, cefixime and potassium tellurite.
A few strains of Escherichia coli O157 are sorbitol-positive and will not be detected directly.
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