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Thiosulfate-Citrate-Bile-Saccharose (TCBS) Agar
Thiosulfate-Citrate-Bile-Sucrose Agar is a selective medium for the isolation of Vibrio cholerae and other enteropathologic Vibrio (in particular Vibrio parahaemolyticus) in fish, seafood and biological samples of animal origin.
The typical composition corresponds to that defined in the standard NF EN ISO 21872-1.
Available as dehydrated medium : 500 g bottle
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
The high concentrations of thiosulfate and sodium citrate, as well as the alkalinity of the medium, considerably inhibit the growth of enterobacteria.
Ox bile and sodium cholate slow the growth of enterococci and inhibit the development of Gram positive bacteria.
The acidification of the medium resulting from the fermentation of sucrose by Vibrio makes bromothymol blue turns yellow.
Using thiosulfate as a sulfur source, the production of hydrogen sulfide is visualized in the presence of ferric citrate. All Vibrio are H2S-negative.
