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Müller-Kauffmann Broth
Müller & Kauffmann Tetrathionate broth is one the oldest media traditionally used for the selective enrichment of Salmonella.
Available as dehydrated base medium 500 g bottle.
Requires Supplement:BS03308 (Novobiocin Selective Supplement) or BS05608
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Ox bile and brilliant green inhibit principally the development of Gram-positive bacteria.
The production of tetrathionate resulting from the action of the iodine-iodide solution on sodium thiosulfate, inhibits coliform bacteria and most intestinal bacteria.
The development of Proteus can be suppressed by adding 40 mg of novobiocin per liter of medium.
Calcium carbonate neutralizes the sulfuric acid produced when tetrathionate is reduced. The resultant effect is to maintain pH at a constant level.
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