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Thioglycollate Broth With Resazurin
Thioglycollate Medium with Resazurin is used for sterility tests of biological products and for the culture of aerobic, anaerobic and microaerophilic bacteria. The formulation of the medium complies with the requirements of the European Pharmacopoeia, the United States Pharmacopoeia and the AOAC for the bacteriological analysis of antibiotics and the determination of the sporicidal effect of disinfectants. This medium is also used for the confirmation of Clostridium perfringens in food products.
The typical composition corresponds to that defined in the European Pharmacopoeia and in the standard NF EN ISO 7937.
Available as dehydrated medium: 500 g bottle.
Also available as ready-to-use medium: 50 tubes of 10 ml (BM08208).
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Pancreatic digest of casein, yeast extract, cystine and glucose assures the growth of a large variety of aerobic and anaerobic bacteria.
Sodium thioglycollate at the concentration of 0.05% decreases the redox potential without having a toxic effect. It also neutralizes the antibacterial power of mercuric derivatives used as preservatives in biological products.
Agar favors the development of anaerobic bacteria by stabilizing the medium against convection currents so anaerobiosis is maintained in the lower part of the recipients.
Resazurin, less toxic than methylene blue, is used as a redox indicator : it is colorless in a reducing medium and becomes pink in an oxidized medium.
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