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EE Broth Acc. To Mossel
EE Broth, buffered and containing bile salts, brilliant green and glucose, proposed by Mossel, is a selective enrichment media for the enumeration by Most Probable Number of Enterobacteriaceae in food, animal and environmental products.
It is also recommended in the various harmonized Pharmacopeia for the detection of Gram negative bacteria that are resistant to bile salts.
The typical composition is that found in the European Pharmacopeia.
Available in dehydrated 500 g bottle.
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
The simultaneous presence of bile salts and brilliant green leads to the inhibition of nearly all Gram positive bacteria as well as Gram negative strains other than enterobacteria.
Pancreatic digest of gelatin and glucose constitute the Nitrogen and energy sources, respectively, for the development of Enterobacteriaceae.
Phosphates allow a suitable buffering of the media in order to increase its capacity for recuperation.
