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Ethyl Violet Azide (Litsky) Broth
Ethyl Violet Azide Broth (also known as Litsky broth) is used to carry out the confirmation test of the detection and enumeration of fecal streptococci (enterococci) in drinking water and waste water, in frozen foods and other food products by the most probable number method. The procedure involves two stages :
- presumptive test in Azide Dextrose Broth (Rothe)
- confirmation in Ethyl Violet Azide Broth (Litsky)
Available in dehydrated medium : 500 g bottle
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Polypeptone and glucose supply the nutrient elements required for the development of enterococci.
The selectivity of the medium for enterococci is due to the presence of ethyl violet and sodium azide, which inhibit the growth of Gram-negative bacilli and sporulated Gram-positive species.
Phosphates buffer the medium and maintain the pH constant.
Sodium chloride maintains the osmotic balance of the medium.
