Lactose-Sulfite (LS) Broth Dehydrated Culture Media Biokar
Biokar

Lactose-Sulfite (LS) Broth

BK140HA
CAD$184.80

Lactose-Sulfite Broth is a confirmation medium allowing the selective detection of both the vegetative cells and spores of Clostridium perfringens in food products and biological samples of animal origin, without the usual confirmatory tests.

The typical composition responds to that defined in the standard NF EN ISO 7937, for the enumeration of Clostridium perfringens in food microbiology.

Available in dehydrated medium: 500 g bottle

Also available as ready-to-use medium: 7 tubes of 9 ml with Durham tube (BM19208)

In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.

QUANTITY

The specificity of the medium to Clostridium perfringens is due principally to the sulfite resistance of microorganism and its ability to ferment lactose with gas production.

The concentration of metabisulfite inhibits the development of the majority of clostridia other than Clostridium perfringens.

Incubation at 46°C assures the specific culture of Clostridium perfringens, which reduces the sodium metabisulfite to sulfide, provoking with ferric citrate a black iron sulfide precipitate that settles in the bottom of the culture tube

BK140HA
Biokar
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