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Modified Trypto-Casein Soy Broth (mTSB)
Modified Tryptone-Soy Broth (mTSB ) is an enrichment medium destined for the detection of pathogenic serotypes of
Escherichia coli, in particular the O157:H7 serotype, in food products and other potentially contaminated samples from animal origin.
The typical composition of the broth corresponds to that defined in the International standard ISO 16654.
Available as: Dehydrated base medium (without novobiocin), 500 g bottle.
To be used with Novobiocin Selective Supplement:
BS05608 – 8 vials of 40 mg
BS03308 – 10 vials of 10 mg
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
The nutrient base combining Tryptone, papaic digest of soybean meal and glucose provides for optimal growth of Escherichia coli O157:H7.
The combined presence of bile salts and novobiocin ensures the inhibition of Gram-positive microorganisms and certain Gram-negative microorganisms such as Proteus.
The dipotassium phosphate maintains pH and thus increases recovery capacity.
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