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Bacillus Cereus Agar (Acc. to Mossel)
Bacillus cereus Agar is used for the detection and enumeration of spores and vegetative cells of Bacillus cereus in food products. It is used in the enumeration at 30°C of presumptive Bacillus cereus according to the standard (NF EN ISO 7932). It is also recommended in the detection or for small numbers method NF EN ISO 21871.
The typical composition corresponds to the MYP agar (mannitol, egg yolk, polymyxin agar) described in the standards NF EN ISO 7932 and NF EN ISO 21871.
Available in the following formats:
Dehydrated base medium : 500 g bottle
Also available as ready-to-use medium: 20 or 120 plates (Ø 90 mm) (BM03808 or BM19908).
Requires Supplement BS05508 (Polymyxine B & Egg Yolk Emulsion) or BS06608 (Egg Yolk Emulsion) AND BS00708 (Polymyxine 5x104 UI)
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Tryptone and meat extract favor the growth of Bacillus cereus.
Sterile egg yolk emulsion is used to detect the presence of lecithinase present in most Bacillus cereus strains.
Insoluble breakdown products of egg yolk lecithin accumulate around the colonies, forming a whitish precipitate.
Mannitol is used to differentiate contaminating microorganisms which ferment it, causing phenol red to turn yellow.
Polymyxin is used to inhibit accompanying microflora when the tested sample is heavily contaminated.
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