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Lauryl Sulfate Tryptose broth
Laurylsulfate-Tryptose broth is a selective enrichment media used for the detection and enumeration of Escherichia coli and coliforms in water and food products. The medium was formulated by Malmann and Darby who showed, in 1941, that sodium laurylsulfate was the best selective agent among a number of surfactants and did not inhibit the growth of coliform bacteria. Levine later showed that the medium reduced the number of false positives by inhibiting the growth of sporulated gas-producing bacteria.
The typical composition responds to that defined in the standards NF T90-413 and NF ISO 7251
Available as dehydrated medium: 500g container.
Also available as ready-to-use medium:
50 tubes of 10 ml (simple strength with Durham tubes) -BM09708
50 tubes of 10 ml (double strength with Durham tubes) -BM09808
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Sodium laurylsulfate considerably inhibits the development of accompanying microbial flora without inhibiting coliform species.
Because of its excellent nutritive capacity, as well as the presence of phosphate buffer, Laurylsulfate-Tryptose Broth enables coliform bacteria to rapidly develop and release large quantities of gas from the fermentation of lactose, even with small inocula.
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