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EMB (Eosin Methylene Blue) Levine Agar
EMB (Eosin Methylene Blue) Agar, Levine is a selective and differentiating medium for isolation and identification of Gram-negative Enterobacteriaceae.
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
EMB (Eosin Methylene Blue) Agar, Levine is a selective and differentiating medium for the isolation and identification of Gram-negative Enterobacteriaceae. Pancreate Digest of Gelatin provides essential nutrients for bacterial growth and Dipotassium Phosphate is the pH buffer. Lactose is the fermentable carbohydrate.
The dyes Eosin Y and Methylene Blue are toxic to most Gram-positive bacteria and serve as pH indicators. Rapid Lactose-fermenting microorganisms produce sufficient quantities of acid to shift the color of the pH indicators to dark purple.
E. coli produces a characteristic green metallic sheen. Non-fecal coliforms cannot ferment Lactose and form normally-colored or colorless colonies on this medium. Agar is the gelifying agent.
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