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m-Green Yeast & Mold Broth
m-Green Yeast & Mold Broth is used for the detection of yeast and fungi in the routine analysis of beverages. Enzymatic Digests of Casein and Animal Tissue provide nitrogenous compounds and carbon sources.
Yeast Extract is a source of B-complex vitamins. Dextrose is the fermentable carbohydrate. Potassium Phosphate serves as buffering agent. Magnesium Sulfate, Thiamine, and Diastase (a mixture containing amylolytic enzymes) provide essential ions, minerals, and nutrients.
The green pH indicator Bromocresol Green diffuses into the colonies. Acidic products from fungal metabolism diffuse into the medium, further lowering the pH and causing the indicator to turn yellow around the colonies. In this medium, the low pH inhibits the growth of bacteria.
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
M-Green Agar for Fungi and Yeasts is a medium used for the detection of yeast and fungi in beverages. This formulation is rich in nutrients which allows for excellent fungal growth.
Casein and gelatin peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Dextrose is the fermentable carbohydrate providing carbon and energy. Bacteriological agar is the solidifying agent. Potassium phosphate is a buffering agent. Magnesium sulfate, thiamine, and diastase (a mixture containing amylolytic (starch) enzymes) provide essential ions, minerals, and nutrients. Bromcresol Green is the pH indicator, facilitating visualization and counting of fungal colonies. The colonies are green due to diffusion of bromcresol green into the colonies.
The end products of the microbial growth diffuse into the medium, reducing the pH and turn the indicator to yellow. Bacterial growth is inhibited by an acid pH. Yeast are large green opaque colonies. Mould appear green and filamentous. Bacteria able to grow at this pH form smaller clear to white colonies.
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