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Pseudomonas Isolation Agar
Pseudomonas Isolation Agar is a modification of a formula described by King, Ward and Raney. It is especially useful in the isolation of Pseudomonas from clinical specimens.
The incorporation of Irgansan selectively inhibits most bacteria but allows the growth of Pseudomonas, and the addition of glycerol enhances pyocyanin production.
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Based on the medium described by King, Ward, and Raney, Pseudomonas Isolation Agar is used for the selective isolation of Pseudomonas and differentiation of Pseudomonas aeruginosa based on pigment production.
Enzymatic Digest of Gelatine provides nitrogenous sources for bacterial growth. The presence of Irgasan allows the growth of Pseudomonas spp. while suppressing that of Gram-positive and some Gram-negative microorganisms.
Magnesium Chloride and Potassium Sulfate enhance the production of the iron chelators pyoverdin (yellow-green or yellow-brown fluorescent) and pyocianin (blue), whose combination produces a characteristic bright green color, by P. aeruginosa. Agar is the gelifying agent.
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