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Phenol Red Mannitol Broth
Phenol Red Mannitol Broth is used in the differentiation of the bacteria by Mannitol fermentation. The nutrient in this formulation is carbohydrate free casein peptone which prevents false positive reactions.
Microorganisms which ferment Mannitol produce acid and change the Phenol Red indicator to yellow. Durham tubes added to the culture medium prior
to sterilization will detect gas production.
Phenol Red Mannitol Broth is a liquid medium recommended to differentiate microorganisms based on their ability to ferment the carbohydrate Mannitol.
Enzymatic Digest of Casein provides additional nutrients (nitrogen and carbon) to the microorganisms, while Sodium Chloride serves as source of essential electrolytes and maintains the osmotic equilibrium.
The pH indicator Phenol Red shifts from red to yellow upon accumulation of acidic metabolic byproducts, thus revealing Mannitol fermentation.
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