- +Products
- +Allergen testing
- +Culture Media - reagents and reference material
- +Environmental testing solutions
- +Laboratory Consumables
- +Laboratory equipment
- +Microbiology equipment
- +Neogen® Food Safety Solutions
- +Temperature & Humidity Monitoring
- Services
- Support
- News
- +About Us
- Contact
Schaedler Broth
Schaedler Broth is a liquid medium for the isolation of anaerobic bacteria from water and clinical samples. Casein, Soy, and Meat Peptones provide nitrogenous compounds essential for bacterial growth.
Yeast Extract is a source for B-complex vitamins and growth enhancer. Dextrose is the fermentable carbohydrate. Hemin stimulates microorganism growth. Sodium Chloride maintains the osmotic equilibrium.
Laked blood may be added to the medium to increase pigmentation of Prevotella and Porphyromonas spp.
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Schaedler Broth is a non-selective medium for cultivating and enumerating anaerobic and aerobic microorganisms. Schaedler Broth was first described by Schaedler et al. as an enriched medium for the isolation of anaerobes from mixed bacteria flora from the gastrointestinal track of mice.
Mata et al., substituting Trypto Soy Broth for casein digest peptone in the Schaedler formulation, found that this medium gave excellent recovery of anaerobic and microaerophilic organisms from human fecal and gastrointestinal specimens.
Schaedler Broth gave the highest rate of growth and response of anaerobic bacteria of nine broth media studied by Stalons et al. General growth requirements are supplied by Trypto Soy Broth, dipeptone, yeast extract and dextrose. Hemin and cystine are added for specific requirements of some anaerobes.
Blood and other enrichments may be added to enhance growth characteristics.
Download
