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EC Medium with MUG
EC Medium with MUG, E05-101, 500g
EC Medium, otherwise known as EC Broth, is a liquid medium used to detect the presence of coliform bacteria in drinking water, wastewater, and foods. Developed by Hajna and Perryl, it was designed to detect coliform bacteria, specifically E. coli. A buffered Lactose broth, it contains Bile Salts to inhibit Gram-positive bacteria and spore-forming microorganisms while enhancing that of E. coli. Coliforms can be detected by growth at 37°C, while Escherichia coli are detected by growth at 44.5°C. Dipotassium Phosphate and Monopotassium Phosphate act as buffering agents while Sodium Chloride maintains the osmotic balance of the medium. The addition of 4-methylumbelliferyl-β-D-glucuronide (MUG) allows for the rapid detection of E. coli. MUG is used as a substrate to detect glucuronidase activity, since its degradation by this enzyme produces the fluorescent compound 4-methylumbelliferone, visible under UV light.
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
EC Medium with MUG may be used for the selective growth of coliform bacteria and for the fluorogenic detection of Escherichia coli in water and food samples.
Casein provides the nutrients for growth and lactose the energy source as a carbohydrate. Sodium chloride maintains osmotic balance, the potassium salts have a buffering capacity, and the bile salts act as a selective agent inhibiting Gram-positive bacteria, bacilli and enterococci while allowing E. coli to grow.
The β-D-glucuronidase of E. coli hydrolyzes MUG and yields a fluorogenic product that can be detected under UV light, thus providing another criterion to determine the presence of this microorganism.
Data sheet
- Volume
- 500 g
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