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Baird-Parker Agar With Egg Yolk Tellurite (RTU)
Baird-Parker Agar with egg yolk and potassium tellurite is a selective medium for the detection and enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) in animal origin biological samples, pharmaceutical products, cosmetics, foods and water.
The typical composition is that defined in the food chain microbiology directives NF EN ISO 6888-1 and NF EN ISO 6888-3.
Available in 20 and 120 plate formats.
Also available in dehydrated powder (BK055HA & BK055GC)
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
The growth of staphylococci is favored by sodium pyruvate and glycine.
Accompanying microflora is inhibited by lithium chloride and potassium tellurite (added extemporaneously), as well as a high concentration of glycine.
The addition of sulfamethazine (optional) after autoclaving inhibits most Proteus and thus limits the invasion of the medium by this species.
Enrichment with egg yolk aids in identification by showing the action of lecithinase.
Staphylococcus aureus presents black or grey colonies (due to the reduction of tellurite to telluride), surrounded by clearing zones in the cloudy egg yolk medium. In principle, other microorganisms are inhibited. However, it is possible to observe brown or greenish micrococci, white yeast colonies or brown Bacillus or Proteus.
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