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Brilliant Green Bile Broth (BGBB) (RTU)
Brilliant Green Bile Broth (BGBB) is used for the confirmation of coliforms and thermotolerant coliforms in food products, water used for watering food products and for effluent / run-off water testing. It can also be used as an enumeration media for coliforms in frozen dairy and ice cream products.
The typical composition of the media responds to that defined in the food microbiology standards NF ISO 4831 and NF ISO 4832. It also conforms to the standards used for water control NF T90-413 and PR NF T90-413.
Available as ready-to-use medium : 50 tubes of 10 ml (simple concentration with Durham tubes).
Also available as dehydrated medium : 500 g bottle (BK002HA).
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
The simultaneous presence of ox bile and brilliant green inhibit almost all Gram-positive organisms and Gram- negative bacteria other than coliforms.
The brilliant green concentration was specifically determined in order to prevent the growth of lactose-fermenting anaerobes at 44°C, which avoids false positives.
Development of coliform bacteria is shown by turbidity and gas production in the Durham tubes, as a result of lactose
fermentation.
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