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King B Agar
King B agar allows the production of fluoresceine (or pyoverdin), a yellow-green pigment that fluoresces under ultraviolet light in certain strains of Pseudomonas. The medium is used primarily in water analysis for the detection and differentiation of Pseudomonas aeruginosa, which produce the characteristic pigment while other species of Pseudomonas do not.
The typical composition responds to that defined in the standard NF EN ISO 16266.
Available as ready-to-use medium: 7 tubes of 7 ml.
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Dipotassium phosphate increases the concentration of phosphorus contributed by the peptone and stimulates the production of fluoresceine, while inhibiting the production of pyocyanin.
Magnesium sulfate contributes the cations necessary for the production of pyoverdin.