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CN Agar For Pseudomonas (RTU)
CN Agar for Pseudomonas is a selective medium for the isolation and enumeration of Pseudomonas aeruginosa in bottled water, swimming pools and water destined for human consumption.
The typical composition of the media responds to the formulation described in the Directives NF EN ISO 16266 and NF T90-421 for water analysis.
Available in ready-to-use medium: 20 or 120 Petri plates Ø 55 mm
Also available in dehydrated medium: 500 g bottle (BK165HA).
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Pancreatic digest of gelatin and acid hydrolyzed casein are the nutrient substrates required for the rapid multiplication of Pseudomonas.
The production of pyocyanin (a blue, non-fluorescent pigment, soluble in water and in chloroform) is stimulated by magnesium chloride and potassium sulfate.
Contaminating yeasts are inhibited by cetrimide.
Nalidixic acid blocks the DNA replication of bacteria sensitive to this antibacterial agent.
Colonies demonstrating a blue-green pigmentation are considered as Pseudomonas aeruginosa.
The other types of colonies are presumptive Pseudomonas aeruginosa and must be confirmed.
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