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Chromogenic Coliform Agar (CCA) (RTU)
Chromogenic Coliform Agar (CCA) is used for the detection and enumeration of Escherichia coli and coliform bacteria in waters of low bacterial numbers (less than 100 total colonies). These waters can be drinking water, disinfected pool water or finished water from treatment plants.
The typical composition complies with the formula described in the standard ISO 9308-1: 2014.
Available as:
Ready-to-use medium: 20 Petri plates Ø 55 mm
Also available as dehydrated medium: 500 g bottle (BK204HA).
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
The simultaneous presence of two substrates enables the detection of the two specific enzyme activities: β- galactosidase and β-glucuronidase.
Coliforms are distinguished by the production of β-galactosidase (β-gal). This enzyme reacts with the chromogenic substrate to produce a pink to red precipitate.
All Escherichia coli possess β-galactosidase and 94 - 97 % are also positive for β-glucuronidase (GUD). The presence of the enzyme is revealed by the presence of a blue coloration. The simultaneous action of the two enzymes gives the colonies of Escherichia coli a purple color.
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