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MSRV Medium (RTU)
Modified Semi-Solid Rappaport-Vassiliadis Agar (MSRV) is a selective medium historically used for the isolation of Salmonella in chocolate and other food products. It is also widely used in animal health: in particular with mammals, poultry and in animal production facilities. It is also recommended for us in the detection of motile Salmonellae in animal fecal matter and in environmental samples in the context of primary animal production.
This medium is not recommended for immobile Salmonellae (Salmonella Gallinarum and Pullorum).
The typical composition responds to that defined in the standards NF EN ISO 6579-1/A1, NF U47-100, NF U47-101 and NF U47-102.
Available in as ready-to-melt medium: 10 vials of 200 ml
Also available as:
- Dehydrated base medium without Novobiocin (BK134HA): 500 g bottle
- Dehydrated medium (BK191HA): 500 g bottle
The Novobiocin supplement is required for BK134HA: BS03308 & BS05608
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Bacteriological efficacy is based on the capacity of salmonellae to selectively migrate in the medium.
The medium is not appropriate for immobile salmonellae (Salmonella Gallinarum, Salmonella Pullorum). If the presence of these strains is suspected, the cultures obtained with preenrichment media must be transferred to appropriate media using conventional standardized procedures.
The high concentration of magnesium chloride and the presence of malachite green inhibit contaminating bacteria.
The addition of novobiocin inhibits most Gram-positive bacteria and avoids the development of Proteus.
Salmonella produce an opaque halo, a reflection of growth.
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