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Lactose-Sulfite (LS) Broth (RTU)
Lactose-Sulfite Broth is a confirmation medium allowing the selective detection of both the vegetative cells and spores of Clostridium perfringens in food products and biological samples of animal origin, without the usual confirmatory tests.
The typical composition responds to that defined in the standard NF EN ISO 7937, for the enumeration of Clostridium perfringens in food microbiology.
Available as ready-to-use medium: 7 tubes of 9 ml with Durham tube.
Also available as dehydrated medium: 500 g bottle (BK140HA).
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
The specificity of the medium to Clostridium perfringens is due principally to the sulfite resistance of microorganism and its ability to ferment lactose with gas production.
The concentration of metabisulfite inhibits the development of the majority of clostridia other than Clostridium perfringens.
Incubation at 46°C assures the specific culture of Clostridium perfringens, which reduces the sodium metabisulfite to sulfide, provoking with ferric citrate a black iron sulfide precipitate that settles in the bottom of the culture tube
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