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Muller-Kauffmann Tetrathionate-Novobiocin Broth (MKTTn) (RTU)
MKTTn Broth is used as one of two selective enrichment medium for the detection of salmonellae following the horizontal method described in the ISO 6579-1 standard. Associated with MSRV medium, it is also used in the protocol for the isolation and identification of Salmonella in animal production environments (NF U 47-100), in poultry (NF U 47-101) and in mammals (NF U 47-102).
MKTTn broth is also used as a second selective enrichment broth for the detection of Salmonella in waters following the protocol described in the NF ISO 19250 standard.
Available: as complet ready-to-use medium: 50 tubes of 10 ml
Also available as :
- Dehydrated medium (without iodine): 500 g bottle (BK208HA)
- Dehydrated medium (without iodine nor Novobiocin): 500 g bottle (BK169HA)
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Bile salts and brilliant green inhibit principally the development of Gram-positive bacteria.
The production of tetrathionate resulting from the action of the iodine-iodide solution on sodium thiosulfate, inhibits coliform bacteria and most intestinal bacteria.
Novobiocin inhibits the development of Proteus.
Calcium carbonate neutralizes the sulfuric acid produced when tetrathionate is reduced. The resultant effect is to maintain pH at a constant level.
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