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Tryptophan Broth (RTU)
Tryptophan broth allows the culture of microorganisms presenting no particular growth requirements. The media is used to perform the indole production test, used notably in the confirmation of Escherichia coli, Shigella and Salmonella.
The typical composition corresponds to that defined in the standards NF EN ISO 9308-1, NF EN ISO 6579-1, NF EN ISO 16654.
Available as ready-to-use medium: 50 tubes of 3 ml
Also available as dehydrated medium: 500 g bottle (BK163HA)
In compliance with regulatory requirements, a USDA permit (VS 16-3) is mandatory for shipments from Canada to the USA due to the inclusion of peptone, an animal byproduct.
Under aerobic conditions, strains possessing a tryptophanase degrade tryptophan to indole.
After culturing the strain, the indole produced is revealed by addition of Kovac’s reagent.