Biokar

Chloramphenicol Selective Supplement

Reference : BS02108

CAD$82.00

The Chloramphenicol Selective Supplement 50 mg, supplied as a lyophilized preparation, inhibits the development of most bacterial contaminants, thereby favoring the isolation of yeasts and molds.

The supplement can substitute for the Oxytetracycline supplement recommended in the routine Directive NF V08- 059, for the enumeration of yeasts and molds in food microbiology. Indeed, when incubation temperatures are higher than 25°C, it is preferable to use chloramphenicol which in these conditions is more stable than oxytetracycline, which loses antimicrobial activity over time.

The supplement can also be added to Sabouraud Agar, (BK025HA), to obtain Sabouraud Chloramphenicol Agar, which also exists in complete dehydrated form (BK007HA).

Freeze-dried supplement: 10 vials of 50 mg.

QUANTITY

Using aseptic techniques, fill the vial with 3 mL of sterile distilled water, then with 2 mL of acetone or with 5 ml of a 1:1 solution ethanol/sterile distilled water.

- Turn end-over-end to dissolve. Avoid frothing the solution.
- Aseptically add 1 ml of the selective supplement to 110 ml of molten Oxytetracycline Glucose base (BK053) held at 44-47°C or to 100 mL Sabouraud Agar (BK025).
- Mix well.

Reference BS02108

Brand: Biokar

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Chloramphenicol Selective Supplement

TS BS021

FT BS021

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